Tlc of sterols steroids and related triterpenoids

Among extracts obtained with method A, only those of N. cataria in dichloromethane (2) and dichloromethane : methanol (3) showed some activity (inhibition diameters of 8–10 mm) against Gram-negative bacteria ( E. coli, Pr. vulgaris, Ps. aeruginosa, Salm. enteritidis and Ser. marcescens ). The petroleum ether extract (1) from H. italicum and P. dodecandra was active against Gram-positive bacteria (inhibition diameters ranged from 12 to 20 mm) while dichloromethane (2) and dichloromethane : methanol extracts (3) from all plants were found to have a low antimicrobial activity. No activity was found with all the methanol extracts (4) against some Gram-positive ( B. subtilis, L. monocytogenes and Staph. aureus ) or Gram-negative bacteria.

The MEP pathway starts with the condensation of pyruvate and D-glyceraldehyde-3-phosphate to 1-deoxy-D-xylulose-5-phosphate (DXP or DOXP). The key isomers DMAPP and IPP are subsequently formed via a series of enzymatic steps starting with the conversion of DXP to 2C-methyl-D-erythritol-4-phosphate (MEP). Enzymes of this MEP pathway are attractive targets for the development of drugs targeting infectious diseases such as malaria and tuberculosis, because this pathway occurs in pathogenic prokaryotes but is absent in human metabolic pathways.

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Tlc of sterols steroids and related triterpenoids

tlc of sterols steroids and related triterpenoids

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tlc of sterols steroids and related triterpenoids

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